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equipment spectramax paradigm multi mode microplate reader  (Danaher Inc)
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plant genotypes seed stocks authentication plants primary human dermal fibroblast cells  (ATCC)
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ATCC plant genotypes seed stocks authentication plants primary human dermal fibroblast cells
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b16f10 murine cell lines  (ATCC)
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ATCC b16f10 murine cell lines
The Gfi1-null microenvironment regulates tumor progression. (A–C) Tumor weight from control (WT Gfi1+/+ or het Gfi1+/−) and Gfi1-null (KO Gfi1−/−) mice analyzed 12–15 days post subcutaneous injection of EL4, LLC1 and <t>B16F10</t> tumor lines. Data are averages ± SD from individual experiments, each representative of 3 performed; (A) EL4 tumors WT/Het n=12; KO n=10; (B) LLC1 tumors WT/Het n=15, KO n=12; (C) B16F10 tumors WT/Het n=12, KO n=10; p values from Student’s t test. (D–G) Monocytes and granulocytes infiltrate tumors from control and Gfi1-null mice. In the bar graphs (D,F), flow cytometry data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3. In the representative flow cytometry plots (E,G), the numerical values are expressed as percentages of total CD11b+ leukocytes in the tumor; p values from Student’s t test. (H,I) Distribution of CD4+ and CD8+ lymphocytes in tumors. The data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3; p values from Student’s t test. (J) Frequency of tumor development in WT mice injected with EL4 cells alone or with splenocytes unfractionated (WT or KO) or depleted of Ly6G+ cells (WT). Splenocytes were from EL4-bearing mice. EL4 alone, EL4+WT cells, EL4+KO cells: n=10; EL4+WT LyG− cells: n=6; WT or KO cells alone: n=3. Data indicate the % mice injected that developed tumors over 14–16 days; p values from Fisher’s exact test. (K) Tumor weight in WT mice injected with LLC1 cell alone or with WT monocytes sorted from bone marrow of LLC1-bearing WT or KO mice; n=6/group. Data are averaged ± SD; p values from Student’s t test.
B16f10 Murine Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plant genotypes seed stocks authentication plants human bone marrow mensenchymal stem cells  (ATCC)
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ATCC plant genotypes seed stocks authentication plants human bone marrow mensenchymal stem cells
The Gfi1-null microenvironment regulates tumor progression. (A–C) Tumor weight from control (WT Gfi1+/+ or het Gfi1+/−) and Gfi1-null (KO Gfi1−/−) mice analyzed 12–15 days post subcutaneous injection of EL4, LLC1 and <t>B16F10</t> tumor lines. Data are averages ± SD from individual experiments, each representative of 3 performed; (A) EL4 tumors WT/Het n=12; KO n=10; (B) LLC1 tumors WT/Het n=15, KO n=12; (C) B16F10 tumors WT/Het n=12, KO n=10; p values from Student’s t test. (D–G) Monocytes and granulocytes infiltrate tumors from control and Gfi1-null mice. In the bar graphs (D,F), flow cytometry data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3. In the representative flow cytometry plots (E,G), the numerical values are expressed as percentages of total CD11b+ leukocytes in the tumor; p values from Student’s t test. (H,I) Distribution of CD4+ and CD8+ lymphocytes in tumors. The data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3; p values from Student’s t test. (J) Frequency of tumor development in WT mice injected with EL4 cells alone or with splenocytes unfractionated (WT or KO) or depleted of Ly6G+ cells (WT). Splenocytes were from EL4-bearing mice. EL4 alone, EL4+WT cells, EL4+KO cells: n=10; EL4+WT LyG− cells: n=6; WT or KO cells alone: n=3. Data indicate the % mice injected that developed tumors over 14–16 days; p values from Fisher’s exact test. (K) Tumor weight in WT mice injected with LLC1 cell alone or with WT monocytes sorted from bone marrow of LLC1-bearing WT or KO mice; n=6/group. Data are averaged ± SD; p values from Student’s t test.
Plant Genotypes Seed Stocks Authentication Plants Human Bone Marrow Mensenchymal Stem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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libani seed extracts  (Cedrus Corporation)
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Cedrus Corporation libani seed extracts
The Gfi1-null microenvironment regulates tumor progression. (A–C) Tumor weight from control (WT Gfi1+/+ or het Gfi1+/−) and Gfi1-null (KO Gfi1−/−) mice analyzed 12–15 days post subcutaneous injection of EL4, LLC1 and <t>B16F10</t> tumor lines. Data are averages ± SD from individual experiments, each representative of 3 performed; (A) EL4 tumors WT/Het n=12; KO n=10; (B) LLC1 tumors WT/Het n=15, KO n=12; (C) B16F10 tumors WT/Het n=12, KO n=10; p values from Student’s t test. (D–G) Monocytes and granulocytes infiltrate tumors from control and Gfi1-null mice. In the bar graphs (D,F), flow cytometry data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3. In the representative flow cytometry plots (E,G), the numerical values are expressed as percentages of total CD11b+ leukocytes in the tumor; p values from Student’s t test. (H,I) Distribution of CD4+ and CD8+ lymphocytes in tumors. The data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3; p values from Student’s t test. (J) Frequency of tumor development in WT mice injected with EL4 cells alone or with splenocytes unfractionated (WT or KO) or depleted of Ly6G+ cells (WT). Splenocytes were from EL4-bearing mice. EL4 alone, EL4+WT cells, EL4+KO cells: n=10; EL4+WT LyG− cells: n=6; WT or KO cells alone: n=3. Data indicate the % mice injected that developed tumors over 14–16 days; p values from Fisher’s exact test. (K) Tumor weight in WT mice injected with LLC1 cell alone or with WT monocytes sorted from bone marrow of LLC1-bearing WT or KO mice; n=6/group. Data are averaged ± SD; p values from Student’s t test.
Libani Seed Extracts, supplied by Cedrus Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plant genotypes seed stocks authentication plants anti  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc plant genotypes seed stocks authentication plants anti
The Gfi1-null microenvironment regulates tumor progression. (A–C) Tumor weight from control (WT Gfi1+/+ or het Gfi1+/−) and Gfi1-null (KO Gfi1−/−) mice analyzed 12–15 days post subcutaneous injection of EL4, LLC1 and <t>B16F10</t> tumor lines. Data are averages ± SD from individual experiments, each representative of 3 performed; (A) EL4 tumors WT/Het n=12; KO n=10; (B) LLC1 tumors WT/Het n=15, KO n=12; (C) B16F10 tumors WT/Het n=12, KO n=10; p values from Student’s t test. (D–G) Monocytes and granulocytes infiltrate tumors from control and Gfi1-null mice. In the bar graphs (D,F), flow cytometry data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3. In the representative flow cytometry plots (E,G), the numerical values are expressed as percentages of total CD11b+ leukocytes in the tumor; p values from Student’s t test. (H,I) Distribution of CD4+ and CD8+ lymphocytes in tumors. The data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3; p values from Student’s t test. (J) Frequency of tumor development in WT mice injected with EL4 cells alone or with splenocytes unfractionated (WT or KO) or depleted of Ly6G+ cells (WT). Splenocytes were from EL4-bearing mice. EL4 alone, EL4+WT cells, EL4+KO cells: n=10; EL4+WT LyG− cells: n=6; WT or KO cells alone: n=3. Data indicate the % mice injected that developed tumors over 14–16 days; p values from Fisher’s exact test. (K) Tumor weight in WT mice injected with LLC1 cell alone or with WT monocytes sorted from bone marrow of LLC1-bearing WT or KO mice; n=6/group. Data are averaged ± SD; p values from Student’s t test.
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genome-wide human 6.0 snp array  (Thermo Fisher)
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Thermo Fisher genome-wide human 6.0 snp array
The Gfi1-null microenvironment regulates tumor progression. (A–C) Tumor weight from control (WT Gfi1+/+ or het Gfi1+/−) and Gfi1-null (KO Gfi1−/−) mice analyzed 12–15 days post subcutaneous injection of EL4, LLC1 and <t>B16F10</t> tumor lines. Data are averages ± SD from individual experiments, each representative of 3 performed; (A) EL4 tumors WT/Het n=12; KO n=10; (B) LLC1 tumors WT/Het n=15, KO n=12; (C) B16F10 tumors WT/Het n=12, KO n=10; p values from Student’s t test. (D–G) Monocytes and granulocytes infiltrate tumors from control and Gfi1-null mice. In the bar graphs (D,F), flow cytometry data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3. In the representative flow cytometry plots (E,G), the numerical values are expressed as percentages of total CD11b+ leukocytes in the tumor; p values from Student’s t test. (H,I) Distribution of CD4+ and CD8+ lymphocytes in tumors. The data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3; p values from Student’s t test. (J) Frequency of tumor development in WT mice injected with EL4 cells alone or with splenocytes unfractionated (WT or KO) or depleted of Ly6G+ cells (WT). Splenocytes were from EL4-bearing mice. EL4 alone, EL4+WT cells, EL4+KO cells: n=10; EL4+WT LyG− cells: n=6; WT or KO cells alone: n=3. Data indicate the % mice injected that developed tumors over 14–16 days; p values from Fisher’s exact test. (K) Tumor weight in WT mice injected with LLC1 cell alone or with WT monocytes sorted from bone marrow of LLC1-bearing WT or KO mice; n=6/group. Data are averaged ± SD; p values from Student’s t test.
Genome Wide Human 6.0 Snp Array, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lines  (ATCC)
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ATCC lines
The Gfi1-null microenvironment regulates tumor progression. (A–C) Tumor weight from control (WT Gfi1+/+ or het Gfi1+/−) and Gfi1-null (KO Gfi1−/−) mice analyzed 12–15 days post subcutaneous injection of EL4, LLC1 and <t>B16F10</t> tumor lines. Data are averages ± SD from individual experiments, each representative of 3 performed; (A) EL4 tumors WT/Het n=12; KO n=10; (B) LLC1 tumors WT/Het n=15, KO n=12; (C) B16F10 tumors WT/Het n=12, KO n=10; p values from Student’s t test. (D–G) Monocytes and granulocytes infiltrate tumors from control and Gfi1-null mice. In the bar graphs (D,F), flow cytometry data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3. In the representative flow cytometry plots (E,G), the numerical values are expressed as percentages of total CD11b+ leukocytes in the tumor; p values from Student’s t test. (H,I) Distribution of CD4+ and CD8+ lymphocytes in tumors. The data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3; p values from Student’s t test. (J) Frequency of tumor development in WT mice injected with EL4 cells alone or with splenocytes unfractionated (WT or KO) or depleted of Ly6G+ cells (WT). Splenocytes were from EL4-bearing mice. EL4 alone, EL4+WT cells, EL4+KO cells: n=10; EL4+WT LyG− cells: n=6; WT or KO cells alone: n=3. Data indicate the % mice injected that developed tumors over 14–16 days; p values from Fisher’s exact test. (K) Tumor weight in WT mice injected with LLC1 cell alone or with WT monocytes sorted from bone marrow of LLC1-bearing WT or KO mice; n=6/group. Data are averaged ± SD; p values from Student’s t test.
Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prorich cathelicidin peptides  (ProRich Seeds)
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The Gfi1-null microenvironment regulates tumor progression. (A–C) Tumor weight from control (WT Gfi1+/+ or het Gfi1+/−) and Gfi1-null (KO Gfi1−/−) mice analyzed 12–15 days post subcutaneous injection of EL4, LLC1 and <t>B16F10</t> tumor lines. Data are averages ± SD from individual experiments, each representative of 3 performed; (A) EL4 tumors WT/Het n=12; KO n=10; (B) LLC1 tumors WT/Het n=15, KO n=12; (C) B16F10 tumors WT/Het n=12, KO n=10; p values from Student’s t test. (D–G) Monocytes and granulocytes infiltrate tumors from control and Gfi1-null mice. In the bar graphs (D,F), flow cytometry data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3. In the representative flow cytometry plots (E,G), the numerical values are expressed as percentages of total CD11b+ leukocytes in the tumor; p values from Student’s t test. (H,I) Distribution of CD4+ and CD8+ lymphocytes in tumors. The data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3; p values from Student’s t test. (J) Frequency of tumor development in WT mice injected with EL4 cells alone or with splenocytes unfractionated (WT or KO) or depleted of Ly6G+ cells (WT). Splenocytes were from EL4-bearing mice. EL4 alone, EL4+WT cells, EL4+KO cells: n=10; EL4+WT LyG− cells: n=6; WT or KO cells alone: n=3. Data indicate the % mice injected that developed tumors over 14–16 days; p values from Fisher’s exact test. (K) Tumor weight in WT mice injected with LLC1 cell alone or with WT monocytes sorted from bone marrow of LLC1-bearing WT or KO mice; n=6/group. Data are averaged ± SD; p values from Student’s t test.
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The Gfi1-null microenvironment regulates tumor progression. (A–C) Tumor weight from control (WT Gfi1+/+ or het Gfi1+/−) and Gfi1-null (KO Gfi1−/−) mice analyzed 12–15 days post subcutaneous injection of EL4, LLC1 and B16F10 tumor lines. Data are averages ± SD from individual experiments, each representative of 3 performed; (A) EL4 tumors WT/Het n=12; KO n=10; (B) LLC1 tumors WT/Het n=15, KO n=12; (C) B16F10 tumors WT/Het n=12, KO n=10; p values from Student’s t test. (D–G) Monocytes and granulocytes infiltrate tumors from control and Gfi1-null mice. In the bar graphs (D,F), flow cytometry data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3. In the representative flow cytometry plots (E,G), the numerical values are expressed as percentages of total CD11b+ leukocytes in the tumor; p values from Student’s t test. (H,I) Distribution of CD4+ and CD8+ lymphocytes in tumors. The data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3; p values from Student’s t test. (J) Frequency of tumor development in WT mice injected with EL4 cells alone or with splenocytes unfractionated (WT or KO) or depleted of Ly6G+ cells (WT). Splenocytes were from EL4-bearing mice. EL4 alone, EL4+WT cells, EL4+KO cells: n=10; EL4+WT LyG− cells: n=6; WT or KO cells alone: n=3. Data indicate the % mice injected that developed tumors over 14–16 days; p values from Fisher’s exact test. (K) Tumor weight in WT mice injected with LLC1 cell alone or with WT monocytes sorted from bone marrow of LLC1-bearing WT or KO mice; n=6/group. Data are averaged ± SD; p values from Student’s t test.

Journal: Cancer research

Article Title: Tumor-infiltrating myeloid cells activate Dll4/Notch/TGF-β signaling to drive malignant progression

doi: 10.1158/0008-5472.CAN-13-3118

Figure Lengend Snippet: The Gfi1-null microenvironment regulates tumor progression. (A–C) Tumor weight from control (WT Gfi1+/+ or het Gfi1+/−) and Gfi1-null (KO Gfi1−/−) mice analyzed 12–15 days post subcutaneous injection of EL4, LLC1 and B16F10 tumor lines. Data are averages ± SD from individual experiments, each representative of 3 performed; (A) EL4 tumors WT/Het n=12; KO n=10; (B) LLC1 tumors WT/Het n=15, KO n=12; (C) B16F10 tumors WT/Het n=12, KO n=10; p values from Student’s t test. (D–G) Monocytes and granulocytes infiltrate tumors from control and Gfi1-null mice. In the bar graphs (D,F), flow cytometry data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3. In the representative flow cytometry plots (E,G), the numerical values are expressed as percentages of total CD11b+ leukocytes in the tumor; p values from Student’s t test. (H,I) Distribution of CD4+ and CD8+ lymphocytes in tumors. The data are expressed as average percentage of total cells from tumor ± SD; EL4: n=5; LLC1: n=6; B16F10 n=3; p values from Student’s t test. (J) Frequency of tumor development in WT mice injected with EL4 cells alone or with splenocytes unfractionated (WT or KO) or depleted of Ly6G+ cells (WT). Splenocytes were from EL4-bearing mice. EL4 alone, EL4+WT cells, EL4+KO cells: n=10; EL4+WT LyG− cells: n=6; WT or KO cells alone: n=3. Data indicate the % mice injected that developed tumors over 14–16 days; p values from Fisher’s exact test. (K) Tumor weight in WT mice injected with LLC1 cell alone or with WT monocytes sorted from bone marrow of LLC1-bearing WT or KO mice; n=6/group. Data are averaged ± SD; p values from Student’s t test.

Article Snippet: Cell culture and in vitro treatments The EL4, LLC1 and B16F10 murine cell lines (from ATCC; authentication confirmed by ATCC through depositor’s analysis of representative cultures from the master seed stock), were propagated in the laboratory for fewer than 6 months in culture medium (RPMI or DMEM with 1% BSA, 2mM L-glutamine, 100IU/ml penicillin, 100μg/ml streptomycin, 50μM 2-ME, and 1mM sodium pyruvate; proliferation was measured by 3 H thymidine incorporation ( 17 ).

Techniques: Control, Injection, Flow Cytometry